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PKD2 (h)

Cat#: 200-0748
Species: Human
RegionRecombinant cells
Enzyme FamilycAMP dependent protein kinase (AGC)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)5E-6
Km (Peptide Substrate)1.0E-04
ATP [M]5E-6
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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