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Pim2 (h)

Cat#: 200-0473
Species: Human
RegionRecombinant cells
Enzyme FamilyCalcium/calmodulin-dependent protein kinase (CAMK)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)3.50E-06
Km (Peptide Substrate)6.6E-05
ATP [M]3.50E-06
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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