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Products and Contract Services

IVIS® Spectrum

Overview


Sign up for our IVIS User Group Meeting on Tuesday, September 9th and Wednesday, September 10th in Nice, France (in conjunction with SMI)! 



The IVIS Spectrum is the newest imaging system available from Caliper Life Sciences. Like previous IVIS systems, it uses Xenogen’s novel patented optical imaging technology to facilitate non-invasive longitudinal monitoring of disease progression, cell trafficking and gene expression patterns in living animals.

The IVIS Spectrum is the most versatile and advanced in vivo imaging system available on the market today. An optimized set of high efficiency filters and spectral un-mixing algorithms lets you take full advantage of bioluminescent and fluorescent reporters across the blue to near infrared wavelength region. It also offers single-view 3D tomography for both fluorescent and bioluminescent reporters that can be analyzed in an anatomical context using our Digital Mouse Atlas.

For advanced fluorescence imaging, the IVIS Spectrum has the capability to use either trans-illumination (from the bottom) or epi-illumination (from the top) to illuminate in vivo fluorescent sources. 3D diffuse fluorescence tomography can be performed to determine source localization and concentration using the combination of structured light and trans illumination fluorescent images. The instrument is equipped with 10 narrow band excitation filters (30nm bandwidth) and 18 narrow band emission filters (20nm bandwidth) that assist in significantly reducing autofluorescence by the spectral scanning of filters and the use of spectral unmixing algorithms. In addition, the spectral unmixing tools allow the researcher to separate signals from multiple fluorescent reporters within the same animal.

For more information in our IVIS® Spectrum Series imaging system please click the button below and complete our product & services inquiry form. 

Request a Quote

or contact David Panzarella at +1-845-735-1921 or David.Panzarella@caliperls.com.




Applications

• Oncology Research (pdf)
• Infectious Disease (pdf)
• Inflammation (pdf)
• Metabolic Diseases (pdf)
• Neurology
• Gene Therapy (pdf)
• Stem cell Biology
• Cardiovascular Disease (pdf)
• Immunology & Transplantation Biology
• Toxicology (pdf)
• Drug Metabolism Studies (pdf)

Features

• High-sensitivity in vivo imaging of fluorescence and bioluminescence
• High throughput (5 mice) with 23 cm field of view
• High resolution (to 20 microns) with 3.9 cm field of view
• Twenty eight high efficiency filters spanning 430 – 850 nm
     - Supports spectral unmixing applications
     - Ideal for distinguishing multiple bioluminescent and fluorescent reporters
• Optical switch in the fluorescence illumination path allows reflection-mode or transmission-mode illumination
• 3D diffuse tomographic reconstruction for both fluorescence and bioluminescence
• Ability import and automatically co-register CT or MRI images yielding a functional and anatomical context for your scientific data.
• NIST traceable absolute calibrations
• Gas anesthesia inlet and outlet ports

Demos

Our product can be demonstrated at our facility in Hopkinton, Massachussetts or other Caliper Life Sciences offices worldwide.  Please contact your local Caliper Representative to arrange a demo.

Specifications

Power Requirements

20 Amps for 120 VAC or 10 Amps for 230 VAC

Dimensions

•Imaging Chamber Interior Size: 43 x 50 x 60 cm (W x D x H) •Imaging System Space Requirement: 203 x 163 x 214 cm (W x D x H)

Camera Sensor

Back-thinned, back-illuminated Grade 1 CCD

CCD

Size 2.7 x 2.7 cm
Operating Temperature -90°C

Imaging Pixels

2048 x 2048

Quantum Efficiency

>85% 500 – 700 nm; >30% 400 – 900nm

Pixel Size

13.5 microns

Minimum Detectable Radiance

70 photons/s/sr/cm2

Field of View (FOV)

Minimum FOV 3.9 x 3.9 cm
Maximum FOV 23 x 23 cm

Minimum Image Pixel Resolution

20 microns

Read Noise

< 3 electrons for bin=1,2,4; < 5 electrons for bin=8,16

Dark Current (Typical)

<100 electrons/s/cm2

Filter Slots

Fluorescence Excitation 12
Fluorescence Emission 24

Fluorescence Filters

Excitation 10
Emission 18

Heated Chamber

Yes

Diffuse Tomography Software

Yes

Gas Anesthesia

Yes

Computer

2.8 GHz, 1 GB RAM, RW CD/DVD, 80 GB HD, 20” flat screen

Lens

f/1 – f/8, 1.5x, 2.5x, 5x, 8.7x magnifications

Stage Temperature

20 – 40°C

Transillumination Stage

Yes

Workbench

Yes