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GSK-3beta (h)

Cat#: 200-0425
Species: Human
RegionRecombinant cells
Enzyme FamilyCyclin dependent kinase containing CDK, MAPK, GSK3, & CLK families (CMGC)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)1.4E-5
Km (Peptide Substrate)1.2E-05
ATP [M]1.4E-5
ReactionFluorescein -peptide + ATP --> fluorescein -phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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