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Sphingosine SK1 (h)

Cat#: 200-0775
Species: Human
RegionRecombinant cells
SubstrateFluorescein Sphingosine
Substrate [M]1.5E-6
Km (ATP)5E-5
Km (Peptide Substrate)2.5E-05
ATP [M]5.00E-05
ReactionFluorescein lipid + ATP --> fluorescein -phospholipid + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
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