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AurA (Aur2/STK6) (h)

Cat#: 200-0412
Species: Human
RegionRecombinant cells
Enzyme FamilyOther
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)2.5E-5
Km (Peptide Substrate)2.0E-06
ATP [M]2.00E-06
ReactionFluorescein -peptide + ATP --> fluorescein phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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