Acetylcholine
| Cat#: | 200-0083 |
| Species: | Rat |
| Region | Forebrain |
| Substrate | Acetylthiocholine |
| Substrate [M] | 0.5E-3 |
| Km (enzyme affinity) | 1.40E-04 |
| Reaction | Acetylthiocholine --> Acetate + Thiocholine / Thiocholine + DTNB (Ellman’s Reagent) --> 5-thio-2-nitrobenzoic acid (yellow) |
| Method | Colorimetric |
| Reference Compound | Physostigmine (Eserine) |
