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Abl (h)

Cat#: 200-0409
Species: Human
RegionRecombinant cells
Enzyme FamilyTyrosine kinase (TK)
SubstrateFluorescein-labeled peptide
Substrate [M]1.5E-6
Km (ATP)4E-6
Km (Peptide Substrate)7.7E-06
ATP [M]4.20E-06
ReactionFluorescein -peptide + ATP --> fluorescein phosphopeptide + ADP
MethodFluorescence – electrophoretic mobility shift
MeasurementSeparation of phosphorylated and unphosphorylated substrate
Reference CompoundStaurosporine
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