4T1-luc2 Bioware® Ultra
Overview
4T1-luc2 is a luciferase expressing cell line which was stably transfected with firefly luciferase gene (luc2). The cell line was established by transducing lentivirus containing luciferase 2 gene under the control of human ubiquitin C promoter.Designation: 4T1-luc2
Tissue: Mouse: mammary gland; adenocarcinoma
Source of Parental Line: ATCC (CRL-2539™)
Gene Transfer Vehicle: pGL4 luc2 Lentivirus
Bioluminescence In Vitro: Approximately 6700 photons/sec/cell. Exact number will vary depending on imaging and culturing conditions.
In vivo Models Tested: Nu/nu (CR); Subcutaneous
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Applications
• Mammary fat pad orthotopic model
• Subcutaneous tumor models
Five 4T1-luc2 cells were injected subcutaneously into the dorsal region near the thigh of female nu/nu mouse. Bioluminescent signal could be detected in the animal way before it could be measured with a caliper. Tumor growth is monitored weekly by an IVIS Spectrum. Tumor was visible 28 days post implantation